diff --git a/change/@fluentui-react-0670c9ba-89d4-45ef-a1a9-e985a92ab991.json b/change/@fluentui-react-0670c9ba-89d4-45ef-a1a9-e985a92ab991.json
new file mode 100644
index 0000000000000..7f80055af0217
--- /dev/null
+++ b/change/@fluentui-react-0670c9ba-89d4-45ef-a1a9-e985a92ab991.json
@@ -0,0 +1,7 @@
+{
+  "type": "patch",
+  "comment": "fix the decision condition in dismissOnTargetWindowBlur to handle the case that clicking inside an iframe.",
+  "packageName": "@fluentui/react",
+  "email": "272097277@qq.com",
+  "dependentChangeType": "patch"
+}
diff --git a/packages/react/src/components/Callout/CalloutContent.base.tsx b/packages/react/src/components/Callout/CalloutContent.base.tsx
index e9d1d2ac206c2..939c33650c8eb 100644
--- a/packages/react/src/components/Callout/CalloutContent.base.tsx
+++ b/packages/react/src/components/Callout/CalloutContent.base.tsx
@@ -342,7 +342,7 @@ function useDismissHandlers(
       if (
         ((preventDismissOnEvent && !preventDismissOnEvent(ev)) ||
           (!preventDismissOnEvent && !preventDismissOnLostFocus)) &&
-        !targetWindow?.document.hasFocus() &&
+        (!targetWindow?.document.hasFocus() || ev.target === targetRef.current) &&
         ev.relatedTarget === null
       ) {
         onDismiss?.(ev);