-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathmissing.html
260 lines (255 loc) · 19.4 KB
/
missing.html
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<title>Metabolomics Workbench File Validator</title>
<link rel="stylesheet" href="styles/styles.css"/>
</head>
<body class="background">
<div>
<h1><a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/" style="text-decoration:none;color:white;">Metabolomics Workbench File Validator</a></h1>
<p>
Last Updated: 2025-03-23 07:40:07.369563
</p>
</div>
<div>
<h2>Statistics</h2>
<p>
Number of Studies: 3<br>
Number of Analyses: 4<br>
</p>
<h3>Validation Statistics</h3>
<div class="stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">mwTab</div>
<div class="stats__grid__item">JSON</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/passing" class="stats__grid__item brightgreen">Passing</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/parsing_error" class="stats__grid__item red">Parsing Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/validation_error" class="stats__grid__item orange">Validation Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/missing" class="stats__grid__item lightgrey">Missing</a>
<div class="stats__grid__item">4</div>
<div class="stats__grid__item">4</div>
</div>
<h3>Comparison Statistics</h3>
<div class="compare__stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">Count</div>
<div class="stats__grid__item brightgreen">Consistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item orange">Inconsistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item lightgrey">Not Checked</div>
<div class="stats__grid__item">4</div>
</div>
</div>
<div>
<h2>File Status</h2>
<div class="grid header">
<a href="#ST003113" class="study__grid__item">ST003113: Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine. - San Raffaele University - Stefanoni, Davide</a>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine.</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Polycystic Kidney Disease (PKD) is a genetic disorder characterized by bilateral cyst formation. We showed that PKD cells and kidneys display metabolic alterations, including the Warburg effect and glutaminolysis, sustained in vitro by the enzyme asparagine synthetase (ASNS). Here, we used antisense oligonucleotides (ASO) against Asns in orthologous and slowly progressive PKD murine models and show that treatment leads to a drastic reduction of total kidney volume (measured by MRI) and a prominent rescue of renal function in the mouse. Mechanistically, the upregulation of an ATF4-ASNS axis in PKD is driven by the amino acid response (AAR) branch of the integrated stress response (ISR). Metabolic profiling of PKD or control kidneys treated with Asns-ASO or Scr-ASO revealed major changes in the mutants, several of which are rescued by Asns silencing in vivo. Indeed, ASNS drives glutamine-dependent de novo pyrimidine synthesis and proliferation in cystic epithelia. Notably, while several metabolic pathways were completely corrected by Asns-ASO, glycolysis was only partially restored. Accordingly, combining the glycolytic inhibitor 2DG with Asns-ASO further improved efficacy. Our studies identify a new therapeutic target and novel metabolic vulnerabilities in PKD. Of interest, in these tracing studies we could confirm that the pyrimidine biosynthesis pathway is increased and rescued by silencing of Asns.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">San Raffaele University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Stefanoni</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Davide</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Via Olgettina 58, Milan, Milan, 20132, Italy</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">[email protected]</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">+393337686005</div>
</div>
</div>
</div>
<div class="grid">
<a href="#AN005098" class="analysis__grid__item lightgrey">AN005098</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005098_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005098</div>
</div>
</div>
<a href="#AN005099" class="analysis__grid__item lightgrey">AN005099</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005099_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005099</div>
</div>
</div>
</div> <br> <div class="grid header">
<a href="#ST003389" class="study__grid__item">ST003389: Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance - Northwest A&F University - Xu, Lei</a>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Metabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Northwest A&F University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Xu</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Lei</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Yangling, Xinong Road, Xiyang, 712100, China</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">[email protected]</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">18149321513</div>
</div>
</div>
</div>
<div class="grid">
<a href="#AN005557" class="analysis__grid__item lightgrey">AN005557</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005557/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN005557_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005557</div>
</div>
</div>
</div> <br> <div class="grid header">
<a href="#ST003744" class="study__grid__item">ST003744: None - None - None, None</a>
<div class="grid__description" style="height:0em;max-height:0">
<div class="desc__grid">
</div>
</div>
</div>
<div class="grid">
<a href="#AN006148" class="analysis__grid__item lightgrey">AN006148</a>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006148/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN006148_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006148/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN006148_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006148/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/docs/validation_logs/AN006148_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN006148</div>
</div>
</div>
</div> <br> </div>
</body>
</html>