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BiocParallel errors #468

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abhijitcbio opened this issue Feb 27, 2025 · 0 comments
Open

BiocParallel errors #468

abhijitcbio opened this issue Feb 27, 2025 · 0 comments

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@abhijitcbio
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Hi,

I am trying to process 18 samples but getting the following error -

Error: BiocParallel errors
  1 remote errors, element index: 1
  17 unevaluated and other errors
  first remote error:
Error in matrix(mcols(exonsByRC)$readCount, dimnames = list(names(exonsByRC), : 'data' must be of a vector type, was 'NULL'
In addition: There were 50 or more warnings (use warnings() to see the first 50)
Execution halted

Here is my code

library(bambu)

gtf.file <- "../../../genome/Homo_sapiens.GRCh38.111.gtf"
fa.file  <- "../../../genome/Homo_sapiens.GRCh38.dna.primary_assembly.fa"
annotations <- prepareAnnotations(gtf.file)

se <- bambu(reads = c(
"../bam/P272_nCD4.bam",
"../bam/P293_nCD4.bam",
"../bam/P295_nCD4.bam",
"../bam/P303_nCD4.bam",
"../bam/P314_nCD4.bam",
"../bam/P317_nCD4.bam",
"../bam/P319_nCD4.bam",
"../bam/P322_nCD4.bam",
"../bam/P332_nCD4.bam",
"../bam/P333_nCD4.bam",
"../bam/P336_nCD4.bam",
"../bam/P342_nCD4.bam",
"../bam/P343_nCD4.bam",
"../bam/P354_nCD4.bam",
"../bam/P358_nCD4.bam",
"../bam/P367_nCD4.bam",
"../bam/P370_nCD4.bam",
"../bam/P373_nCD4.bam"
), annotations = annotations, genome = fa.file, discovery = TRUE, quant = TRUE, lowMemory = TRUE, ncore = 1, verbose = TRUE)

writeBambuOutput(se, ".", prefix="nCD4_transcriptome")
saveRDS(se, paste0("nCD4_transcriptome.rds"))

For your reference, I have attached the full log.

Looking forward to your help.

log.txt

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